Mutations which block the binding of calmodulin to Spc110p cause multiple mitotic defects
نویسندگان
چکیده
منابع مشابه
Pcp1p, an Spc110p-related calmodulin target at the centrosome of the fission yeast Schizosaccharomyces pombe.
In the budding yeast Saccharomyces cerevisiae, the calmodulin-binding protein Spc110p/Nuf1p facilitates mitotic spindle formation from the fungal centrosome or spindle pole body (SPB). The human Spc110p orthologue kendrin is a centrosomal, calmodulin-binding pericentrin isoform that is specifically overexpressed in carcinoma cells. Here we establish an evolutionary and functional link between S...
متن کاملMutations in yeast calmodulin cause defects in spindle pole body functions and nuclear integrity
Yeast calmodulin (CaM) is required for the progression of nuclear division (Ohya, Y. and Y. Anraku. 1989. Curr. Genet. 15:113-120), although the precise mechanism and physiological role of CaM in this process are unclear. In this paper we have characterized the phenotype caused by a temperature-sensitive lethal mutation (cmdl-101) in the yeast CaM. The cmdl-101 mutation expresses a carboxyl-ter...
متن کاملThe spacer protein Spc110p targets calmodulin to the central plaque of the yeast spindle pole body.
Yeast calmodulin (CaM) was found to be localized to the microtubule organizing centre, the spindle pole body. The spindle pole body is a multi-layered structure consisting of outer, central and inner plaques. In this paper, we report that a fraction of CaM is in association with the central plaque of the spindle pole body. This localization is dependent on the calmodulin-binding site of another...
متن کاملMutations in Hnrnpa1 cause congenital heart defects
Incomplete penetrance of congenital heart defects (CHDs) was observed in a mouse model. We hypothesized that the contribution of a major genetic locus modulates the manifestation of the CHDs. After genome-wide linkage mapping, fine mapping, and high-throughput targeted sequencing, a recessive frameshift mutation of the heterogeneous nuclear ribonucleoprotein A1 (Hnrnpa1) gene was confirmed (Hnr...
متن کاملMultiple instance learning of Calmodulin binding sites
MOTIVATION Calmodulin (CaM) is a ubiquitously conserved protein that acts as a calcium sensor, and interacts with a large number of proteins. Detection of CaM binding proteins and their interaction sites experimentally requires a significant effort, so accurate methods for their prediction are important. RESULTS We present a novel algorithm (MI-1 SVM) for binding site prediction and evaluate ...
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ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 1996
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.109.6.1297